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Mobile phone microscopes have detected a "silent-sensitive" honey bee

Use the portable parasitic sensor portable device. Credit: UCLA Institute of Technological Advancement Technology

Nurses are among the leading makers of global food supply pollination, and contributions to the US have a value of $ 15 to $ 20 million a year. The rapid decline in honey bees colonies in the global, and in particular in the United States, has intensified the future tension of agricultural pollination and food security. Parasites form one of the factors affecting the decline of bee population, most commonly found with Nosema ceranea and Nosema apis. Nosema Apisen infections may have symptoms of honey dysentery and defecation at the beehive entrance, but Nosema ceranae infection does not show physical symptoms. Honey bees "silent silence" infects bees, drones and bees, and can also cause colony collapse.

Currently, this is the only way to diagnose this disease by detecting spores of the parasite through a clear microscope. However, this traditional test is carried out in laboratory settings and requires the operation of the experts. For this reason, the beekeeper must send local samples to the remote laboratory for a specific diagnosis, which is time consuming and costly.

UCLA researchers at the School of Engineering Samueli, in collaboration with the Barnard College Biology Department, have developed a mobile microscope to detect honey nosema spores and quickly automate them in field settings. A mobile and cost-effective platform, the only 0.8-pound weighing machine, is a mobile-based microscope-based microscope, a personalized mobile phone application and sample preparation protocol that lets fluorescence tags into parasitic bees.

Aydogan Ozcan, UCLA's Chancellor of Electrical and Computer Engineering and the Associate Director of the California NanoSystems Institute at UCLA, undertook research with Jonathan Snow, Assistant Professor at the Barnard College (NY) and Hatice Ceylan Koydemir, Senior Researcher at UCLA. Research was published Lab chip, Magazine of the Royal Society of Chemistry (United Kingdom).

The diagnostic diagnosis of this new platform involves the preparation of the sample, where honey bees are removed and half-dissected, and a small amount of stain is added to the fluorescent spores of the parasite. A drop of prepared solution is placed in a glass slide, then it will be added to the mobile microscope for analysis. The image of the sample is then captured by the smartphone and transmitted to a computer through automated analysis, so that spores count rapidly, which is less than 90 seconds to be sent to the user.

The researchers demonstrated cellular performance performance and analyzed the samples and is able to detect the concentration of the parasite underneath the threshold that is necessary to advise the parasite treatment device. That is why this mobile device uses the planned sensitivity to establish a treatment.

"Ensuring that bees' welfare is a major problem in food safety and ecosystem stability is the fact that there are many factors that affect the decline of bee populations, parasitic infections with important roles, this mobile device develops to tackle this new problem in an inexpensive way, And, our knowledge, the first and only platform Nosema Spores is in the field of implementation. " said Ozcan.

North American Pollinators Protection Campaign sponsored by the National Science Foundation Engineering Research Center (ERC) and the Howard Hughes Medical Institute (HHMI).

Explore more:
The tiny parasite helps reduce the honey-bee colony by infecting the larvae

More information:
Jonathan W. Snow et al. The images of the bees Nosema ceranae esporas spores quickly, detect and quantify using a mobile fluorescence microscope, Chip Lab (2019). DOI: 10.1039 / C8LC01342J

Magazine reference:
Chip Lab

UCLA Institute of Technological Advancement Technology

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